Generic algorithm to predict the speed of translational elongation: implications for protein biogenesis.

Generic algorithm to predict the speed of translational elongation: implications for protein biogenesis.

Blog Article

Synonymous codon usage and variations in the level of isoaccepting tRNAs exert a powerful selective force on translation fidelity.We have developed an algorithm to evaluate the relative rate of translation which allows large-scale comparisons of the non-uniform translation rate on the protein biogenesis.Using the complete genomes of Escherichia coli and Bacillus subtilis usssa pro m softballs we show that stretches of codons pairing to minor tRNAs form putative sites to locally attenuate translation; thereby the tendency is to cluster in near proximity whereas long contiguous stretches of slow-translating triplets collar twojeys are avoided.The presence of slow-translating segments positively correlates with the protein length irrespective of the protein abundance.

The slow-translating clusters are predominantly located down-stream of the domain boundaries presumably to fine-tune translational accuracy with the folding fidelity of multidomain proteins.Translation attenuation patterns at highly structurally and functionally conserved domains are preserved across the species suggesting a concerted selective pressure on the codon selection and species-specific tRNA abundance in these regions.